January 2021, Vol. 6, No. 1, pp. 8-14.
The effect of Solvent on the Phytochemical and Antimicrobial activity of Arachis hypogaea Leaf
A. A. Alafy¹*, D. Kubmarawa¹, F. D. Davuram¹, S. Gwani², M. Akwaras³
¹Department of Chemistry, School of Physical and Applied Sciences, Modibbo Adama University of Technology, Yola, Adamawa State, Nigeria.
²Department of Microbiology, Microbiology Laboratory Unit, Gombe State University,
Gombe State, Nigeria.³Department of Veterinary and Science Laboratory Technology, Federal college of Animal and Health Production Technology, Vom, Plateau State, Nigeria.
*Corresponding author’s e-mail:email@example.com
Arachis hypogaea is one of the most common widely consumed leguminous crops in Africa. The leaf is widely used because of its antioxidant, anti-inflammatory and antimicrobial tendencies. The effect of solvent on the Phytochemical and antimicrobial activity of Arachis hypogaea was carefully examined in this research work using ethanol, n-Hexane, ethyl-acetate and aqueous as extracting solvent. Standard methods were adopted for the Phytochemical and antimicrobial activity. The Phytochemical results shows the presence of almost all the secondary metabolite tested for such as Alkaloids, Flavonoids, Saponins, Carbohydrate and Tannins in all the four crude extract except for ethanolic extract where Flavonoids was found to be absent, so also Glycoside, Phytosterols, Steroids, Phenols, and Anthraquinones were also found to be absent in the Aqueous extract. The antimicrobial activity of the plant ethanolic, n-Hexane, etyl-acetate and Aqueous crude extract was carried out against Escherichia coli, Shigella dysenteriae, Salmonella typhi, Klebsiella pneumoniae, Helicobactea pylori, Pseudomonas aeruginosa, Streptococcus aureus and were all found to be reasonably active and effective against the tested isolate, however ethanol and aqueous solvents are better extracting medium for the Phytochemical and antimicrobial activity of Arachis hypogaea than the rest of the solvents used as this gives reason for the continual consumption of Arachis hypogaea as food and for medicinal purposes.
Keywords:Arachis hypogaea; Antimicrobial activity; Phytochemical analysis; Microorganism.
- Nostro A, Germanò MP, D'angelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol 2000;30:379-84.
- Rates, SMK. Plants as source of drugs. Toxicon 2001;39:603–13.
- Food and Agriculture Organization. Trade in medicinal plants. In: Economic and Social Department, Food and Agriculture Organization of the United Nations, Rome. 2004;2-3.
- Alafy AA, Davuram FD, Kubmarawa D, Okechukwu JO, Emmanuel IV. Antioxidant Activities and Phytochemical Screening of Peanut (Arachis hypogea) Leaves. African Journal of Environment and Natural Science Research 2020;3(5):28-37.
- Kubmarawa D, Ajoku GA, Enwerem NM, Okorie DA. Preliminary phytochemical and antimicrobial screening of 50 medicinal plants from Nigeria. Afr J Biotechnol 2007;6:1690-6.
- Sofowora AE. Medicinal Plants and Traditional Medicines in Africa. Screening plants for Bioactive agents. 2nd edition. Sunshine house, Ibadan, Nigeria: Spectrum Books Lmt; Ibadan, Nigeria.1993; 289:134-156.
- Harbone JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Chapman and Hall Ltd. London. 1973;279.
- Trease GE, and Evans WC. Pharmacognosy. 15th Ed. London :saunders publishers; 2002.
- Mitscher LA, Leu RP, Balhala MS, Beal JI, White R. Antimicrobial agents from higher plants. Introduction, rational and methodology. Liayadia. 1972;35:157-66.
- Ali NA A, Julich WD, Kusnick C, Lindequist U. Screening of Yemeni medicinal plants foranti bacterial and cytotoxicactivities. J Ethnophamacol 2001;74:173-9
- Ogunwade IA. Composition patterns of the essential oils of the leaves of Eucalyptus, Thuja, Callitris & Melaleuca species growing in Nigeria. PhD.Thesis Department of chemistry university of Nigeria. 2001.
- Kubmarawa D, Kidah M.I, Shagal MH. Antimicrobial Activities of Essential oils from some medicinal and Aromatic Plants. British Biotechnology Journal. 2016;14(3):1-6.
- Prabasheela B, Venkateshwari R, Nivetha S, Priya P, Karthik K. phytochemical analysis and antioxidant activity of the ethanolic extract three different forms of Arachis hypogea. International Journal of Food Science and Nutrition 2015;3(2):100-21.
- Shagal MH, Kubmarawa D, Alim H. Preliminary phytochemical investigation and antimicrobial evaluation of roots, stem-bark and leaves extracts of Diospyros mespiliformis. International Research Journal of Biochemistry and Bioinformatics 2012;2(1);11-5
- Dahiru.D.,Malgwi A.R., Sambo H. S. Growth inhibitory effect of Senna siamea leaf extracts on selected microorganisms. American Journal of Medicine and Medical Science 2013;3(5):103-7.