International Journal of Modern Science and Technology
August 2018, Vol. 3, No. 8, pp 181-189.
Nanobiocomposite of fungal asparaginase and magnetic nanoparticle: Synthesis, Characterization and Anticancer Activity against MCF-7 and HT-29 Cancer cell lines
G. Baskar*, Garrick Bikku George
Department of Biotechnology, St. Joseph’s College of Engineering, Chennai – 600 119. India.
*Corresponding author’s e-mail: firstname.lastname@example.org
Nanobiotechnology is a new era paving a way to develop advanced technology especially in the field of medicine. In the present work, magnetic nanobiocomposite of fungal asparaginase was produced using polyethylene glycol. The formation of magnetic nanobiocomposite of asparaginase was confirmed by laser light scattering and confirmed using UV-Visible spectrophotometer. The specific activity of fungal asparaginase was increased from 320 U/mg of crude asparaginase to 385.81 U/mg of magnetic nanobiocomposite. The primary amines majorly involved in binding of asparaginase on magnetic nanoparticls were confirmed using FT-IR analysis. The AFM analysis has revealed the size of magnetic nanobiocomposite in the range of 130 to 160 nm. The synthesized magnetic nanobiocomposite has shown good cytotoxicity on MCF-7 and HT-29 cancer cell lines. Hence synthesized magnetic nanobiocomposite of fungal asparaginase could be used as an active anticancer drug.
Keywords: Fungal asparaginase; Nanobiocomposite; Anticancer drug; Cancer cells; Magnetic nanoparticles; Characterization.
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