International Journal of Modern Science and Technology

INDEXED IN 

ISSN 2456-0235

​​​​​​​​​​​​​August 2018, Vol. 3, No. 8, pp 173-180. 

​​​Consolidated Bioprocessing in Solid State Fermentation for the Production of Bioethanol from a Novel Mixture of Surgical Waste Cotton and Waste Cardboard

R. Navnit kumar, T. R. Sambavi, S. Renganathan*
Centre for Biotechnology, A.C.Tech., Anna University, Chennai-600 025. India.

​​​*Corresponding author’s e-mail: renganathansaha@gmail.com

​Abstract

​Consolidated bioprocessing to simultaneously produce cellulolytic enzymes, saccharify the biomass and ferment the released monosaccharides to ethanol is a much sought after technique in the vast area of biomass to ethanol conversion. In the present work, 100 g of a mixture of surgical waste cotton and waste card board (1:1) ratio were subjected to CBP in a solid state fermentation mode. Yeast was grown for 48 h as sheets on 4 large nutrient agar petri-dishes (10 cm diameter). The (10% w/v optimized) yeast sheets were laid on the bottom sieve of the SSF aerated tray reactor. Over the yeast sheets were spread 100 g of surgical waste cotton and waste card board mixture. 4 filter papers (15 cm diameter) inoculated and incubated with T. harzanium for 5 days were laid over the bed of the cellulosic mixture. After 12 days of incubation for CBP in a static humidity chamber at 30-32°C (optimized), around 11.25 ± 0.1 g/L ethanol with a percentage cellulose conversion of 11.04% was produced and 30 ± 0.02 g/L glucose was left unconsumed. The enzyme activities at the end of 12 days were FPases: 2.4 ± 0.09 IU/mL, CMCases: 16.8 ± 0.1 IU/mL, Xylanases: 936.15 ± 0.1 IU/mL and Beta-glucosidases: 3917.5 ± 0.09 IU/mL.

Keywords: Consolidated Bioprocessing; Cellulolytic enzymes; Solid State Fermentation; Bioethanol.

​References

  1. ​​Schneider WDH, Reis LD, Camassola M, Dillon AJP. Morphogenesis and Production of Enzymes by Penicillium  echinulatum in Response to Different Carbon Source. BioMed Research International. 2014;2014:Article ID 254863.
  2. Hansen GH, Lübeck M, Frisvad JC, Lübeck PS, Andersen B. Production of cellulolytic enzymes from ascomycetes: Comparison of solid state and submerged fermentation. Process Biochemistry 2015;50(9):1327-41.
  3. Ferreira V, Faber MO, Mesquita SS, Pereira Jr N. Simultaneous saccharification and fermentation process of different cellulosic substrates using a recombinant Saccharomyces cerevisiae harbouring the β-glucosidase gene. Electronic Journal of Biotechnology 2010;13(2):1-7.
  4. Olson DG, McBride JE, Shaw AJ, Lynd LR. Recent progress in consolidated bioprocessing. Current opinion in Biotechnology 2011;23:1-10.
  5. Gholamreza Salehi Jouzani, Mohammad J, Taherzadeh. Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review. Biofuel Research Journal. 2015;5:152-95.
  6. Mohamed HALA, Haggag WM. Mutagenesis and inter-specific protoplast fusion between Trichoderma koningii and Trichoderma reesei for biocontrol improvement. American Journal of Scientific and Industrial Research 2010; 1(3):504-15.
  7. Aslam N, Shiekh MA, Ashraf M, Jamil A. Expression pattern of Trichoderma Cellulases under Different Carbon Sources. Pakistan Journal of Botany 2010;42(4):2895-902.
  8. Lima MA, Lavorente GB, da Silva HKP, Bragatto J, Rezende CA, Bernardinelli OD, de Azevedo ER, Gomez LD, McQueen-Mason SJ, Labate CA, Polikarp I. Effects of pretreatment on morphology, chemical composition and enzymatic digestibility of eucalyptus bark: a potentially valuable source of fermentable sugars for biofuel production – part 1. Biotechnology for Biofuels 2013;6(75):75-91.
  9. Rajaa ASM, Arputharaj A, Saxena S, Patil PG. Single bath enzymatic scouring and bleaching process for preparation of absorbent cotton. Indian Journal of Fibre and Textile Research 2017;42:202-8.
  10. Ma Y, Hummel M, Maattanen M, Sarkilahti A, Harlin A, Sixta H. Upcycling of waste paper and cardboard to textiles. RSC Advances journal. 2015; 18: 858-866.
  11. Xia L, Cen P. Cellulase production by solid state fermentation on lignocellulosic waste from the xylose industry. Process Biochemistry 1998;39(9):909-12.
  12. Ghose TK. Measurement of cellulase activities. Pure and Applied Chemistry 1987;59(2):257-68.
  13. Batista AS,    Miletti LC, Stambuk BU. Sucrose Fermentation by Saccharomyces cereviseae lacking Hexose Transport. Journal of Molecular Microbiology and Biotechnology 2004;8(1):26-33.
  14. André L, Cavalett RA, Lima A. Enhancement of Escherichia coli cellulolytic activity by co-production of beta-glucosidase and endoglucanase enzymes. Electronic Journal of Biotechnology 2010;13(5):1-9.
  15. Nogueira KMV, Costa MN, de Paula RG, Natividade FCM, Azevedo RR, Silva RN. Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose. BMC Microbiology 2015:15(195):1-12.
  16. Bakri Y, Akeed Y, Thonart P. Comparison between Continuous and Batch Processing to Produce Xylanase By Penicillium canescens 10-10c. Brazilian Journal of Chemical Engineering 2012; 29(3):441-8.
  17. Choudhary J, Saritha M, Nain L, Arora A. Enhanced Saccharification of Steam-Pretreated Rice Straw by Commercial Cellulases Supplemented with Xylanase. Journal of Bioprocess and Biotechnology 2016;4(188):1-6.
  18. Singh N, Mathur AS, Tuli DK, Gupta RP, Barrow CJ, Puri M. Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring. Biotechnology for Biofuels 2017;10(73):1-18.
  19. Brethauer S, Studer MH. Consolidated bioprocessing of lignocellulose by a microbial consortium. Energy and Environmental Science 2014;7(4):1446-53.