International Journal of Modern Science and Technology
November 2018, Vol. 3, No. 11, pp. 230-237.
Phytochemical Analysis of Mitracarpus villosus and Comparative Toxicity of Mitracarpus villosus Ointment and Honey
J. A. Jato*, I. Bawa, F. N. Onyezili
Department of Biochemistry, University of Agriculture Makurdi, P.M.B 2373, (970001) Makurdi, Nigeria.
*Corresponding author’s e-mail: email@example.com
Studies on compounds present in plant or animal matter helps in identifying compounds responsible for harmful and beneficial effect in plants and animal by-products. This study was designed to identify and quantify phytochemical compounds in Mitracarpus villosus methanolic leaf extract, and determine the toxicity of M villosus ointment and honey. The results show that M. villosus leaves contains phenolics, saponins, flavonoids, cardiac glycosides and tannins but no alkaloids. Quantitative phytochemical analysis showed varied percentage content of these compounds with saponins being highest (14.0%) and tannins lowest (1.41%). Acute oral toxicity was determined to be 15066 mg/kg and 7542 mg/kg as LD50 for honey and M. villosus ointment respectively, inferring that both honey and M. villosus ointment belong to the nontoxic class group of substances. Primary irritation indices were recorded as 0.16 and 0.33 for honey and ointment respectively. Therefore, honey and M. villosus ointment belong to the category of negligible irritants. These findings is indicative of the promising potentials of M. villosus ointment as a topical remedy for diseases and further confirms reasons for high demand of honey for various uses despite its alarming cost increase.
Keywords: Mitracarpus villosus; Ointment; Honey; toxicity; Phytochemical.
- Khaliqur R, Arshad H, Shafqat U, Imdad UMZ. Phytochemical Analysis and Chemical Composition of Different Branded and Unbranded Honey Samples. International Journal of Microbiological Research 2013;4(2):132-37.
- Alvarez-Suarez JM, Gasparrini M., Forbes-Hernández TY, Mazzoni L, Giampieri F. The Composition and Biological Activity of Honey: A Focus on Manuka Honey. Foods 2014;3:420-32. doi:10.3390/foods3030420
- Nisbet HO, Nisbet C, Yarim M, Guler A, Ahmet O. Effects of three types of honey on cutaneous wound healing. Wounds 2010; 22(11):275-83.
- Obioma BLE, Okwesili FCN, Victor NO. Therapeutic effect of honey bee venom. Journal of Pharmaceutical, Chemical and Biological Sciences 2016;4(1):48-53.
- Aboh MI, Olayinka BO, Adeshina GO, and Ibrahim K. Preliminary studies on the antifungal activities of the successive extracts of Mitracarpus villosus (Sw.) Dc aerial parts obtained in Abuja, Nigeria. Malaysian Journal of Microbiology 2014; 10(2):133-38.
- Adamu A, Musa D, Egharevba HO. Bioactive Chemical Compounds and Pharmacological Prospect of Mitracarpus villosus (Swartz) DC- a review. Asian Journal of Biochemical and Pharmaceutical Research 2016;2(7):99-113.
- Adamu A, Oibiokpa IF, Musa D, Inobeme A. Phytochemical Constituents and Antimicrobial Activity of Some Medicinal Plants Used for Treating Skin Diseases in Bosso Local Government, Niger State, Nigeria. Journal of Complementary and Alternative Medical Research 2017;3(3): 1-9.
- Arhewoh MI, Eichie FE, Iwuagwu UN. AntiFungal Activity and Stability of a Cream Formulation of Mitracarpus villosus (Rubiaceae) Extract. African Journal of PharmaceuticalResearch and Development 2011;3(1):38-42.
- Rodrigo LF, Richard MG, Lidiane OD, Elaine SC, Gabriele MMC, Elaine MD, Adilson DD, Elita S. Antitumor, Antibiotic and Antileishmanial Properties of the Pyranonaphthoquinone Psychorubrin from Mitracarpus frigidus. Anais da Academia Brasileira de Ciências. 2012;84(4):1081-89.
- Ekpendu TO, Akah PA, Adesumoju AA, Okogun JI. Antiinflammatory and antimicrobial activities of Mitracarpus scaber extracts. Pharma Biol 1994;32(2): 191-96.
- Udegbunam SO, Nnaji TO, Udegbunam RI, Okafor JC, Agbo I. Evaluation of herbal ointment formulation of Milicia excelsa (Welw) C.C, berg for wound healing. African Journal of Biotechnology 2013;12(21):3351-59.
- Auwal SM, Atiku MK, Wudil AM, Sule MS. Phytochemical Composition and Acute Toxicity Evaluation of Aqueous Root Bark Extract of Securidaca longipedunculata (Linn). Bayero Journal of Pure and Applied Sciences 2012;5(2): 67-72.
- Rao USM, Abdurrazak M, Khamsah SM. Phytochemical Screening, Total Flavonoid and Phenolic Content Assays of Various Solvent Extracts of Tepal of Musa paradisiaca. Malaysian Journal of Analytical Sciences 2016;20(5):1181-90.
- Tarannum A, Shamsi S, Zaman R. Acute Toxicity of Polyherbal Unani Formulation Habbe Shifa in Experiemntal animal models Indian Journal of traditional Knowledge. 2014; 13(1): 171-174
- OECD, guideline for testing of chemicals-402, Acute Dermal Toxicity. 1987.
- Fawehinmi AB, Hassan L, Etatuvie SO, Oyedeji FO. Preliminary phytochemical screening and antimicrobial evaluation of four medicinal plants traditionally used in Nigeria for skin infection. African Journal of Pure and Applied Chemistry 2013;7(2: 44-49.
- Inbathamizh L, Padmini E. Effect of Geographycal Location on the Phytochemical Composition and Antioxidant Potential of Moringa oleifera Flowers. BioMedRx 2013;1(3):239-247.
- OECD, Organization for Economic Co-operation and Development. Guideline for testing of chemicals-423, Documentation on Acute Oral Toxicity and Acute Class Method. 2001.
- Okunji EK, Musa G. and Ali AJ. Toxicological Evaluation of Five Herbal Drugs Hawked In Minna, Niger State Journal of Applied Pharmaceutical Science 2012;2(12):167-71.
- Ado AB, Oloro J, John OJ. Acute and Sub-Acute Toxicity Study of Ethanolic Crude Extract of Mitracarpus hirtus Plant on Wistar Rats. International Journal of Science and Healthcare Research 2017;2(4):1-11.
- Samat S, Nor NA, Hussein FN, Ismail WIW. Short-Term Consumption Of Gelam Honey Reduces Triglyceride Level. International Food Research Journal 2017; 24(4):1519-24.
- Jansen SA, Kleerekooper I, Hofman ZLM, Kappen IFPM, Stary-Weinzinger A, Van der Heyden MAG. Grayanotoxin Poisoning: ‘Mad Honey Disease’ and Beyond. Cardiovasc Toxicol 2012; 12:208-15.
- Al-Salihi KA, Al-Rammahi HM. Diarrhea Associated with Ingestion of Zygophyllum coccineum (Tartir) in Camels in the Al-Najaf Desert in Iraq. International Journal of Pharmacognosy and Phytochemical Research 2014;3:1-6.
- Draize JH, Woodard G, Calvery HO. Methods For the Study of Irritation and Toxicity of Substances Applied Topically to the Skin and Mucous Membranes. J Pharmacol Exp Ther 1944;82:377-90.
- Djerrou Z. Djaalab H, Riachi F, Serakta M, Chettoum A, Maameri Z, Boutobza B, Hamdi-Pacha Y. Irritantcy Potential and Sub-Acute Dermal Toxicity Study Of Pistacia Lentiscus Fatty Oil As A Topical Traditional Remedy. Afr J Tradit Complement Altern Med 2013;10(3):480-89. http://dx.doi.org/10.4314/ajtcam.v10i3.15.
- Manyi-Loh CM, Clarke AM, Ndip RN. An overview of honey: Therapeutic properties and contribution in nutrition and human health. African Journal of Microbiology Research 2011;5(8):844-52.