ISSN 2456-0235

International Journal of Modern Science and Technology

INDEXED IN 

​​​​​​​​International Journal of Modern Science and Technology, Vol. 2, No. 3, 2017, Pages 98-104. 


Anticancer Activity of Iron oxide Nanobiocomposite of Fungal Asparaginase

G. Baskar, J. Chandhuru, A. S. Praveen, K. Sheraz Fahad
Department of Biotechnology, St. Joseph’s College of Engineering, Chennai – 600 119. India. 
*Corresponding author’s e-mail: basg2004@gmail.com

Abstract
Drug delivery using nanoparticles has been found to be more effective compared to normal drug delivery and has many advantages. Iron oxide nanoparticles synthesis is of greater importance because targeting the drug to the desired location is brought by applying magnetic field, which is of lesser cost. In the present work iron oxide nanobiocomposite was produced using L-asparaginase rich culture filtrate produced from Aspergillus terreus. Synthesized iron oxide nanobiocomposite was characterized using UV-Vis spectrum, X-Ray Diffraction (XRD), Fourier Transform Infra-Red (FTIR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray (EDX) analysis. An absorption peak at 330 nm confirmed the formation of iron oxide nanoparticles by fungal culture filtrate with asparaginase. The presence of functional groups like O-H, C=O, C-H and C=N on surface of the iron oxide nanobiocomposite was confirmed by FT-IR analysis. The XRD analysis proved that the synthesized nanobiocomposites are crystalline in nature. Produced nanobiocomposites were in the size range of 40-100 nm. The EDX analysis confirmed the presence of iron and oxygen in the synthesized nanobiocomposite. The MTT assay was carried to study the Anti-cancerous nature of the synthesized nanobiocomposites on MCF-7 cell line.  MTT assay showed that decrease in cell viability, the viability of the cells decreased to 41.9% when it was treated with iron oxide nanobiocomposite of asparaginase. Thus it is proved that the iron oxide nanobiocomposite of asparaginase is a potential anticancer drug. 

​​Keywords: Nanobiocomposite; Characterization; L-asparaginase; Anticancer activity; Drug delivery.

References

  1. Feynman R. There's plenty of room at the bottom. Science. 1991;254:1300-1301.
  2. Salata OV. Applications of nanoparticles in biology and medicine. J Nanobiotechnol. 2004;2:3.
  3. Mazzola L. Commercializing nanotechnology. Nature Biotechnology. 2003;21:1137-1143.
  4. Boisselier E, Astruc D. Gold nanoparticles in nanomedicine: preparation, imaging, diagnostics, therapies and toxicity. Chem Soc Rev. 2009;38:1759-1782.
  5. Faraji, AH, Wipf P. Nanoparticles in cellular drug delivery. Bioorganic and Medicinal Chemistry. 2009;17:2950-2962.
  6. Gopal, M,  Gogoi R,  Srivastava C,  Kumar R, Singh PK, Nair KK, Yadav S, Goswami AVA. Nanotechnology and its application in plant protection, Plant Pathology in India: Vision 2030, Indian Phytopathological Society. New Delhi. 2011. pp.224-230.
  7. Sivakumar T. Properties and Applications of Nanoparticles–Review. Int J Adv Res Biol Sci 2014;1:251-260.
  8. Lee H, Messersmith PB. Bio-Inspired Nanomaterials for a New Generation of Medicine, Nanotechnology in Biology And Medicine: Methods, Devices, and Applications (Ed. Tuan Vo-Dinh), CRC Press, Boca Raton, FL, 2006.
  9. Pathak P, Katiyar VK. Multi-Functional Nanoparticles and Their Role in Cancer Drug Delivery – A Review, AZojomo (Journal of Material online), 2007; DOI : 10.2240/azojono0114.
  10. Pal T, Pal A, Panigrahi S. Bimetallic Nanoparticles: Synthesis and Characterization, Nanotechnology in Biology And Medicine: Methods, Devices, and Applications (Ed. Tuan Vo-Dinh), CRC Press, Boca Raton, FL, 2006.
  11. Kaittanis C, Shaffer TM, Daniel LJ, Thorek DLJ, Grimm J. Dawn of Advanced Molecular Medicine: Nanotechnological Advancements in Cancer Imaging and Therapy. Crit Rev Oncog. 2014;19:143-176.
  12. Jha RK, Jha PK, Chaudhury K, Rana SVS, Guha SK. An emerging interface between life science and nanotechnology: present status and prospects of reproductive healthcare aided by nano-biotechnology. Nano Reviews. 2014;5:10.3402/nano.v5.22762.
  13. Whiteley C, Govender Y, Riddin T, Mahendra Rai M. Enzymatic Synthesis of Platinum Nanoparticles: Prokaryote and Eukaryote Systems, Metal Nanoparticles in Microbiology (Ed. Mahendra Rai, Nelson Duran). 2011:103-134.
  14. Baskar G, Chandhuru J, Fahad KS, Praveen AS. Mycological synthesis, characterization and antifungal activity of zinc oxide nanoparticles, Asian J Pharm Tech. 23(4);2013:142-146.
  15. Baskar G, Chandhuru J, Sheraz Fahad K, Praveen AS, Chamundeeswari M, Muthukumar T. Anticancer activity of fungal l-asparaginase conjugated with zinc oxide nanoparticles. J Mater Sci Mater Med. 26;2015:1-5.
  16. Baskar G, Renganathan S. Optimization of L-asparaginase production by Aspergillus terreus MTCC 1782 using response surface methodology and artificial neural network linked genetic algorithm. Asia-Pac J Chem Eng. 2012;7:212-220.
  17. Baskar G, Chandhuru J, Praveen AS, Sheraz Fahad K. Synthesis and Characterization of Gold Nanobiocomposite of Asparaginase using Aspergillus terreus. International Journal of Modern Science and Technology. 2016;1(8)288-295.
  18. Wriston JC Jr, Yellin TO. L-asparaginase-a review. Adv Enzymol Relat Areas Mol Biol. 1973;39:185-248.
  19. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55-63.

ISSN 2456-0235