ISSN 2456-0235

International Journal of Modern Science and Technology

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Extraction, characterization and biological applications of Secondary Metabolites of Endophytic Fungi of Codium variegatum

​A P Amsapraba, K S Janane, Gurunathan Baskar*
Department of Biotechnology, St. Josephs College of Engineering, Chennai 600119, India.
*Email: basg2004@gmail.com

​​Abstract
Many chemical substances produced by some fungi find application in various spheres and therefore draw scientific interest. Among such fungi, the ones living in plant tissue attract particular attention. Codiaeum variegatum was selected as a host plant because of unique bioactive compounds produced by its fungal colonizers. In order to discover them, it was decided not to skip stages and conduct the study thoroughly. As a preliminary stage, fungi collection had to be conducted followed by cultivation in conditions where both temperature and light remain stable. For isolation of secondary metabolites of the studied fungal isolates, alcohol was used first followed by fractional separation using planar and column chromatography where each fraction had to be clearly identified. Further, the composition of obtained fractions was analyzed. The final stage consisted of bioassay. In order to find out about the composition of metabolites, techniques such as UV-Vis, FTIR and GC-MS were used. In order to determine the biological activities of discovered secondary metabolites, tests for free radical neutralization, inhibition of α-amylase and antibacterial activity were performed. Among the tested compounds, Tris (2, 4-di-tertbutylphenyl) phosphate was found which was known before for antioxidant properties. Under infrared spectroscopy examination, characteristic bands corresponding to OH groups, double bonds of carbon and phenyl rings could be observed. As amounts increased, so did radical cleanup power; enzyme interference grew stronger, bacterial growth slowed in clear patterns. From start to finish, findings point toward tiny fungi tucked inside C. variegatum leaves holding steady promise not loud claims for future drug-linked discoveries.

Keywords: Endophytic fungi, C. variegatum, Secondary metabolites, Anti-diabetic, Antibacterial activities.

​References

  1. Ye G, Huang C, Li J, Chen T, Tang J, Liu W, Long Y. Isolation, structural characterization and antidiabetic activity of new diketopiperazine alkaloids from mangrove endophytic fungus Aspergillus sp. 16-5c. Mar Drugs 2021;19(7):402. https://doi.org/10.3390/md19070402
  2. Lagashetty A, Anusha M, Rachana, Channabasavaraja M, Shivakumar, Veena V, Preeti RK, Ganiger SK. Exploring potential biological applications of green derived silver nanoparticles using Codiaeum variegatum leaf extract. Next Res 2024;100103. https://doi.org/10.1016/j.nexres.2024.100103
  3. Mollick AS, Shimoji H, Denda T, Yokota M, Yamasaki H. Croton Codiaeum variegatum (L.) Blume cultivars characterized by leaf phenotypic parameters. Sci Hortic (Amsterdam) 2012;133:1–8. https://doi.org/10.1016/j.scienta.2011.09.038
  4. Deshmukh SK, Gupta MK, Prakash V, Saxena S. Endophytic fungi: a source of potential antifungal compounds. J Fungi 2018;4(3):77. https://doi.org/10.3390/jof4030077
  5. Sudha V, Govindaraj R, Baskar K, Al-Dhabi NA, Duraipandiyan V. Biological properties of endophytic fungi. Braz Arch Biol Technol 2016;59:e16150436. https://doi.org/10.1590/1678-4324-2016150436
  6. Schulz B, Boyle C, Draeger S, Römmert AK, Krohn K. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 2002;106(9):996–1004. https://doi.org/10.1017/S0953756202006342
  7. Sieber TN. Endophytic fungi in forest trees: are they mutualists? Fungal Biol Rev 2007;21(2–3):75–89. https://doi.org/10.1016/j.fbr.2007.05.004
  8. Noor S, Begum MN, Rony SRR, Chowdhury AA, Sohrab MH, Mazid MA. Bioactivity and chemical screening of endophytic fungi associated with seaweeds Gracilaria sp. and Sargassum sp. of the Bay of Bengal, Bangladesh. Sci Rep 2025;15:16121. https://doi.org/10.1038/s41598-025-00099-y
  9. Cherif W, Ktari L, Hassen B, Ismail A, El Bour M. Indigenous species Codium fragile ssp. fragile: identification, characterization, and biotechnological potential. Microorganisms 2024;12(9):1803. https://doi.org/10.3390/microorganisms12091803
  10. Karthikeyan A, Venkatesan G, Sampathkumar P. Exploring marine fungal diversity and their applications in agriculture. In: Prasad R, editor. Microbial Biotechnology: Basic Research and Applications. Singapore: Springer; 2022. p. 293–310. https://doi.org/10.1007/978-981-16-8877-5_13
  11. Kaur T, Kalia A. Endophytic fungi: biodiversity, ecological significance, and potential industrial applications. In: Yadav AN, Singh S, Mishra S, Gupta A, editors. Recent Advancement in White Biotechnology Through Fungi. Cham: Springer; 2019. p. 1–62. https://doi.org/10.1007/978-3-030-10480-1_1
  12. Ancheeva E, Daletos G, Proksch P. Bioactive secondary metabolites from endophytic fungi. Curr Med Chem 2020;27(11):1836–1854. https://doi.org/10.2174/0929867326666190916144709
  13. Kusari S, Hertweck C, Spiteller M. Chemical ecology of endophytic fungi: origins of secondary metabolites. Cell Chem Biol 2012;19(7):792–798. https://doi.org/10.1016/j.chembiol.2012.06.004
  14. Kusari S, Hertweck C, Spiteller M. Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites. Phytochem Rev 2012;11:487–505. https://doi.org/10.1007/s11101-012-9260-6
  15. Zheng R, Li S, Zhang X, Zhao C. Biological activities of some new secondary metabolites isolated from endophytic fungi: a review study. Int J Mol Sci 2021;22(2):959. https://doi.org/10.3390/ijms22020959
  16. Jha P, Kaur T, Chhabra I, Panja A, Paul S, Kumar V, Malik T. Endophytic fungi: hidden treasure chest of antimicrobial metabolites interrelationship of endophytes and metabolites. Front Microbiol 2023;14:1227830. https://doi.org/10.3389/fmicb.2023.1227830
  17. Vasundhara M, Sudhakara Reddy M, Kumar A. Secondary metabolites from endophytic fungi and their biological activities. In: Gupta VK, editor. New and Future Developments in Microbial Biotechnology and Bioengineering: Microbial Secondary Metabolites Biochemistry and Applications. Amsterdam: Elsevier; 2019. p. 237–258. https://doi.org/10.1016/B978-0-444-63504-4.00018-9
  18. Li X, Zhang Y, Wang Y, Chen L. Research advances on endophytic fungi and their bioactive metabolites. Bioprocess Biosyst Eng 2023;46:165–170. https://doi.org/10.1007/s00449-022-02840-7
  19. Zhang HW, Song YC, Tan RX. Novel secondary metabolites from endophytic fungi: synthesis and biological properties. Phytochem Rev 2020;19:425–448. https://doi.org/10.1007/s11101-020-09672-x

International Journal of Modern Science and Technology 2026;11(1):1-14.                                     Full Paper (PDF)