ISSN 2456-0235

International Journal of Modern Science and Technology

INDEXED IN 

International Journal of Modern Science and Technology 2025;10(1):1-11.                                            Full Paper (PDF)

Hydrolysis of waste rice water using immobilized α-Amylase for production of nutritional energy drink

​Rajamurugan Shivani, Soundararajan Aniesha, Gurunathan Baskar*
Department of Biotechnology, St. Josephs College of Engineering, Chennai 600119, India.
*Corresponding author: basg2004@gmail.com

​​Abstract
Rice water, a nutrient-rich byproduct of cooking, acts as a low-cost substrate for value-added applications. In the present work, rice water starch was hydrolyzed using α-amylase immobilized on zinc oxide nanoparticles, enabling efficient glucose conversion. A maximum glucose yield of 6.2 mg/ml was obtained under optimized conditions (0.08% enzyme, 45°C, 20 min). The resulting glucose-rich hydrolysate was evaluated for its potential as an energy drink base due to its high glucose content and nutritional value.  Further fermentation of the hydrolysate was carried out by Saccharomyces cerevisiae.  The characterization of the fermented hydrolysate identified the presence of valuable bioactive compounds such as 2,2-dimethyl-4-hexen-3-one, 2,6,10-dodecatrienoic acid (7,11-dimethyl-3-(trifluoromethyl)), 2,2,4-trimethylpentane (isooctane), and oxalic acid derivatives. These compounds are known for their potential uses in food, pharmaceutical, and industrial applications. Thus, this study demonstrated an innovative approach to converting food waste into functional beverages and bioactive ingredients, supporting circular bioeconomy and sustainable development goals.

​​Keywords: Rice water hydrolysate, Enzyme immobilization, Energy drink, Bioactive compounds.

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