International Journal of Modern Science and Technology

INDEXED IN 

ISSN 2456-0235

International Journal of Modern Science and Technology, 1(7), 2016, Pages 242-249. 


Sequences of Luciferase Enzyme from Extracted 25 Organisms to Create Database using WAMP Software 

R. P. Sasikala, K. S. Meena
Department of Chemistry, Bioinformatics Infrastructure Facility Centre,Queen Mary’s College, University of Madras, Chennai – 600 004. India.

Abstract
Luciferases are the enzymes that catalyze the reactions producing light. The oxidative mechanism which leads to light emission is similar for most luciferases. However, these enzymes and their substrates are evolutionarily unrelated. The firefly enzyme luciferase catalyzes the luminescent reaction of luciferin with ATP and oxygen. This substance is only slowly transported through cell membranes, in contrast to the aldehyde substrate in the bacterial reaction. Therefore the bacterial luciferase system seems more suitable to the study of environmental or developmental changes in gene expression in living cells.The property of bioluminescence identified in various bacteria, fireflies and insects has been illustrated in detail in this database. The sequences of luciferase enzyme from 25 organisms have been extracted from databases in Genbank and fasta format. Those sequences were analyzed for its physical and chemical parameters and also for identifying homologous sequences. The databases have been created using WAMP software which includes Windows, Apache, MySQL, PHP.

​​Keywords: Luciferase; Database; WAMP; Sequence; Enzyme; PHP. 

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