International Journal of Modern Science and Technology
August 2020, Vol. 5, No. 8, pp. 204-209.
Effect of methanolic leaf extract of Stachytarpheta jamaicensis on weight and packed cell volume of mice experimentally infected with Trypanosoma brucei brucei
E. J. Udo¹*, C. O. Ali², D. E. Oboho³, T. S. Atsuwe⁴
¹Department of Biological Sciences, Faculty of Science, Nigerian Defence Academy, Kaduna.
²Department of Zoology, College of Science, Federal University of Agriculture Makurdi, Benue State, Nigeria.
³Department of Animal and Environmental Biology, University of Uyo, Uyo Akwa Ibom State
⁴Department of Zoology, College of Science, Federal University of Agriculture Makurdi, Benue State, Nigeria.
*Corresponding author’s e-mail: email@example.com
Trypanosoma brucei brucei has detrimentally affected livestock and livestock production. The main means of controlling this disease is by the use of synthetic trypanocides but they are unsafe, unreliable because of reports of their toxic effect, and parasite resistant. Thus, the study was aimed at evaluating the effect of leaf of Stachytarphyta jamaicensis on the Weight and Packed Cell Volume (PCV) of mice experimentally infected with T. brucei brucei. Leaves of S. jamaicensis were extracted using methanol solvent. Thirty (30) mice weighing between 25-40g were divided into 6 groups (group 1- 6) of 5 mice each. Each Mouse was inoculated with 0.2 ml of blood containing an absolute number (2.0 x 106) of T. brucei brucei parasites (blood diluted with normal saline to 2 parasites per field / ml intramuscularly/mouse) and were treated with various concentrations of 100, 250 and 500mg/bw of methanol leaf extract of S. jamaicensis. Diminazine (Nozomil®) at standard concentration of 3.5mg/bw was used as control. Data collected were analyzed using analysis of variance (ANOVA) at p<0.05 level of significance. Result showed that treatment with methanol leaf extract of S. jamaicensis resulted in significant increase (p<0.05) in weight of the treated mice. There was also a continuous decrease in the PCV values of all the groups of mice infected but treated with the methanol leaf extract of S. jamaicensis but this was significantly higher (P<0.05) when compared with the infected untreated group. S. jamaicensis extract has demonstrated efficacy, which has suggested its anti-trypanosomal properties. It can however be considered as an alternative in the absence of synthetic chemical drugs.
Keywords:Stachytarphyta jamaicensis; Packed cell volume; Methanol leaf extract; Anti-trypanosomal property; Trypanosoma brucei brucei.
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