International Journal of Modern Science and Technology
International Journal of Modern Science and Technology, Vol. 2, No. 2, 2017, Pages 50-55.
Ethanol Determination using Immobilized Enzymes
K. Vidhyarthy¹, S. Chittibabu²,*
¹Department of Biotechnology, Periyar Maniammai University, Vallam, Thanjavur- 613 403, Tamil Nadu, India.
²Department of Biotechnology & Chemical Engineering, Periyar Maniammai University, Vallam, Thanjavur- 613 403, Tamil Nadu, India.
*Corresponding author’s e-mail: firstname.lastname@example.org
Several analytical methods have been developed during the years for use in food, forensic, fuels, clinical, and pharmaceutical industries. These include the use of chemical, spectroscopic and chromatographic methods. Though these methods are precise and reliable, they are complex, expensive and time consuming. Such drawbacks could be overcome by the use of enzymatic methods. Enzymes are highly specific biological catalysts and find a widespread application in biosensors. Their nature, specificity and catalytic properties make them excellent tools for chemical analysis. Biosensors based on the enzymes such as alcohol oxidase and horseradish peroxidase are intended to be discussed in this review, concerning their applications and detection mechanism in determination of compounds.
Keywords: Enzymes; Biosensors; Alcohol oxidase; Horseradish peroxidase.
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