International Journal of Modern Science and Technology
International Journal of Modern Science and Technology, 1(7), 2016, Pages 242-249.
Sequences of Luciferase Enzyme from Extracted 25 Organisms to Create Database using WAMP Software
R. P. Sasikala, K. S. Meena
Department of Chemistry, Bioinformatics Infrastructure Facility Centre,Queen Mary’s College, University of Madras, Chennai – 600 004. India.
Luciferases are the enzymes that catalyze the reactions producing light. The oxidative mechanism which leads to light emission is similar for most luciferases. However, these enzymes and their substrates are evolutionarily unrelated. The firefly enzyme luciferase catalyzes the luminescent reaction of luciferin with ATP and oxygen. This substance is only slowly transported through cell membranes, in contrast to the aldehyde substrate in the bacterial reaction. Therefore the bacterial luciferase system seems more suitable to the study of environmental or developmental changes in gene expression in living cells.The property of bioluminescence identified in various bacteria, fireflies and insects has been illustrated in detail in this database. The sequences of luciferase enzyme from 25 organisms have been extracted from databases in Genbank and fasta format. Those sequences were analyzed for its physical and chemical parameters and also for identifying homologous sequences. The databases have been created using WAMP software which includes Windows, Apache, MySQL, PHP.
Keywords: Luciferase; Database; WAMP; Sequence; Enzyme; PHP.
- Belobrov PI, Denisov IA, Tumanyan AG, Esimbekova E, Meshajkina LV, Yakimov AS, Kratasyuk VA. Luciferase-based biobarcode amplification assay, Proceedings of the 10th International Conference on Modern Problems of Radio Engineering, Telecommunications and Computer Science, Lviv-Slavske, 2010 pp.372.
- Bernengo JC, Brau F, Steghens JP. ATP measurements on single living cells a dynamic approach. Engineering in Medicine and Biology Society (1996) 1907-1908.
- Cai D, Marques MA, Nogueira F. Full color modulation of firefly luciferase through engineering with unified Stark effect. The Journal of Physicalchemistry 117 (2013) 13725-13730.
- Evans MS, Chaurette JP, Adams ST Jr, Reddy GR, Paley MA, Aronin N, Prescher JA, Miller SC. A synthetic luciferin improves bioluminescence imaging in live mice. Nat Methods 11 (2014) 393-395.
- Fukushima M, Kataoka T, Sugiyama N, Mohri K. Firefly luciferin-luciferase luminescence by milligauss ultra-low frequency pulsed magnetic fieldapplied pure water without ATP. Magnetics Conference (2005) 1151-1152.
- Manninen T, Ribeiro A, Lloyd-Price J, Linne ML, Ruohonen K , Yli-Harja O, Kauffinan SA. Parameter Estimation and Tuning of FireflyLuciferase Pathway Model. Proceedings of the 5th IEEE International Workshop on Genomic Signal Processing and Statistics (GENSIPS 2007), IEEE Signal Processing Society, Tuusula, Finland, 2007 pp.1-4.
- Muthukumaran T, Krishnamurthy NV, Sivaprasad N, Sudhaharan T. Isolation and characterization of luciferase from Indian firefly, Luciola praeusta. The Journal of Biological and Chemical Luminescence 29 (2014) 20-28.
- Katsuhiko N. Thermal behaviour of luciferase nanofabricated hydrophobic Si surface. Biomacromolecules 9 (2008) 1081-1083.
- Katsuhiko N, Tadatsugu H. Heat shock structure of luciferase on wet-treated Si surface. Journal of Applied Physics 106 (2009) 54702-54702.
- Vieira J, Pinto da Silva L, Esteves da Silva JC. Advances in the knowledge of light emission by firefly luciferin and oxyluciferin. Journal of Photochemistry and Photobiology 117 (2012) 33-39.
- Xiang W, Qiushu C, Yuze S, Xudong F. Bio-inspired optofluidic lasers with luciferin. Applied Physics Letters 102 (2013) 203706.
- Inouye S, Sato J, Sahara-Miura Y, Yoshida S, Hosoya T. Luminescence enhancement of the catalytic 19kDa protein (KAZ) of Oplophorus luciferase by three amino acid substitutions. Biochemical and Biophysical Research Communications 445 (2014) 157-162.
- Kheirabadi M, Sharafian Z, Naderi-Manesh H, Heineman U, Gohlke U, Hosseinkhani S. Crystal structure of native and a mutant of Lampyris turkestanicus luciferase implicate in bioluminescence color shift. Biophysics 1834 (2013) 2729-2735.
- Langridge W, Escher A, Wang G, Ayre B, Fodor I, Szalay A. Low-light image analysis of transgenic organisms using bacterial luciferase as a marker. Journal of Bioluminescence and Chemiluminescence 9 (1994) 185-200.
- Cheraghi R, Hosseinkhani S, Davoodi J, Nazari M, Amini-Bayat Z, Karimi H, Shamseddin M, Gheidari F. Structural and functional effects of circular permutation on firefly luciferase: in vitro assay of caspase. International Journal of Biological Macromolecules 58 (2013) 336-342.
- Fan F, Keith V. Wood. Bioluminescent Assays for High-Throughput Screening. Assay and Drug Development Technologies 5 (2007) 127-136.
- Wood KV, Lam YA, Seliger HH, McElroy WD. Complementary DNA coding click beetle luciferases can elicit bioluminescence of different colors. Science 244 (1989) 700-702.
- Verhaegent M, Christopoulos TK. Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization. Anal Chem 74 (2002) 4378-4385.
- Markova SV, Golz S, Frank LA, Kalthof B, Vysotski ES. Cloning and expression of cDNA for a luciferase from the marine copepod Metridia longa. A novel secreted bioluminescent reporter enzyme. J Biol Chem 279 (2004) 3212-3217.
- Thompson EM, Nagata S, Tsuji FI. Cloning and expression of cDNA for the luciferase from the marine ostracod Vargula hilgendorfii. Proc Natl Acad Sci USA 86 (1989) 6567-6571.